郑 静 副教授

　　电话：021-64250608
    Email：zhengjing@ecust.edu.cn

简历

1997-2001  南开大学 生化与分子生物学系  学士

2001-2003  中科院上海生物化学与细胞生物学研究所  硕士

2003-2008  中科院上海神经科学研究所  博士

2008-至今  华东理工大学药学院   博士后研究，现任副教授

       郑静博士具有分子生物学、肿瘤生物学、生物化学和神经生物学等多方面的技术背景和研究经验，负责并主持过多项神经系统疾病和肿瘤领域的项目申请与执行，如上海市浦江计划、科技部863项目、国自然青年基金、上海市自然基金等。已经在Carcinogenesis，Journal of Neuroscience, Journal of Biological Chemistry, Neurobiology of Disease, J Neurooncol.，Neuroscience，Brain Research Bulletin，AJTR等学术刊物上发表论文二十余篇，被SCI他人引用近百余次。研究方向如下所述：

l  神经系统智力障碍疾病发生发展的分子机制与药物靶标开发     

 智力发育障碍又称为精神发育不全或弱智，是儿童期最常见的神经系统疾病之一。引起智力障碍的原因可以是遗传性的，如多基因或单基因突变；也可以是非遗传性的，如神经系统感染，药物滥用，或脑损伤。其主要症状是智力发育迟缓，适应能力低下，注意力不集中等。部分病人（20-30%）伴随有癫痫发作。智力障碍的发病率约为2-3% 。在欧洲，约有8% 的医疗费用于智力疾病的治疗，远远高于其他同类疾病的费用。因此，智力障碍疾病成为医学领域中最重要的有待解决的难题之一。在小鼠中选择性的在特定脑区敲除与智力障碍疾病相关的基因，通过分析突触可塑性、学习记忆和癫痫发生等指标研究这些基因的功能；小鼠特定脑区中在单个神经元水平敲除与智力障碍疾病相关的基因，并且使该神经元可视化，从而研究这些基因在神经元形态学、发育和可塑性中的作用。上述工作陆续发表在2015和2011年度的Neuroscience ，2014年度的Brain Research Bulletin ，2013年度的PNAS，2008年度的J. Biol. Chem.，2010年的Neurobiology of Disease，2011年的Int J Clin Exp Path上。 
 

l  神经系统肿瘤发生发展的分子机制与药物靶标开发      

胶质瘤是一种常见的脑肿瘤，发生于神经外胚层。按细胞种类分，胶质瘤的主要类型有星形胶质细胞瘤，少突胶质细胞瘤和胶质母细胞瘤。2007年，世界卫生组织按胶质瘤的恶性程度和预后的好坏情况将胶质瘤分为I、II、III、IV，四个等级。I级胶质瘤通常是良性的，在儿童中发病更频繁，一般不会发展为更高级别肿瘤，其基因变化也与更高级别的胶质瘤不同，预后良好。II级属分化良好性，虽然不是良性的，但预后较好，中位生存期为5-8年。III级是渐变性星形胶质细胞瘤，中位生存期为3年。IV级的胶质母细胞瘤，恶性最强，中位生存期为12-18个月，大于60岁患者的生存期低于中位生存期。我们利用CRISPR/Cas9基因编辑技术、RNA干扰技术和基因过表达/点突变技术等，结合肿瘤细胞生长曲线、克隆形成、细胞周期、细胞凋亡、细胞迁移、血管生成、裸鼠皮下成瘤和原位成瘤等检测技术，研究我们关注的药靶基因对神经系统肿瘤发生和发展的影响，以期望寻找到药物作用的靶标，开发基于基因治疗和药物治疗的新产品。上述工作陆续发表在2015年度的AJTR，2014年度的Carcinogenesis和Int J Clin Exp Path上，2013年度的J Neurooncol.，2012年度的J Am Chem Soc.，2010年的J. Neurosci.和2011和2015年度的ACS Nano上。

l  病毒介导的基因转导工具的开发和生物反应器产业化应用研究          

AAV（腺相关病毒）和Lentivirus（慢病毒）基因转导工具载体的开发和专利申请、产业化应用以及转化医学基础研究，相关技术和专利陆续获得多家公司和临床单位的资助和推广。

   1.         Wu Congyu, Wang Chong, Zheng Jing, Luo Chao, Li Yanfang, Guo Shouwu, Zhang Jingyan. Vacuolization in Cytoplasm and Cell Membrane Permeability Enhancement Triggered by Micrometer Sized Graphene Oxide. ACS Nano, 2015, accepted. (IF=12.033)

2.         Zhang Ling, Zheng Jiawei, Zheng Jing*. STAT3 is a key pathway in primary intraosseous squamous cell carcinoma arising from an odontogenic keratocyst.American Journal of Translation Research, accepted. (IF=3.863)

3.         Wang Hong-Tao, Zeng Lin, Chen Qian, Zhang Xin, Liu Ji-Wei, Lu Ting-Jia, Xiong Zhi-Qi, Zheng Jing*, Hu Ze-Lan*: β-Catenin is required for maintaining hippocampal morphology during the perinatal period. Neuroscience, 2015. 284:273-282. (IF=3.327)

4.         Li Kui, Liu Ji-Wei, Zhu Zhi-Chuan, Wang Hong-Tao, Zu Yong, Liu Yong-Jie, Yang Yan-Hong, Xiong Zhi-Qi, Shen Xu, Chen Rui, Zheng Jing*, Hu Ze-Lan*. DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test. International Journal of Clinical and Experimental Pathology, 2014. 7(10): 6486-6492. (IF=1.783)

5.         Liu Yong-Jie, Zhu Zhi-Chuan, Xiong Zhi-Qi, Zheng Jing*, Hu Ze-Lan, Qiu Jiang-Feng. Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells. International Journal of Clinical and Experimental Pathology, 2014. 7(9):5750-5761. (IF=1.783)

6.         Zhu Zhi-Chuan, Liu Yong-Jie, Li Kui, Liu Ji-Wei, Wang Hong-Tao, Sun Bing, Xiong Zhi-Qi, Jiang Hua-Liang, Zheng Jing*, Hu Ze-Lan*. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility. Carcinogenesis, 2014. 35(8):1901-1910. (IF=5.266)

7.         Huang Bo, Lu Yi-Sheng, Li Xia, Zhu Zhi-Chuan, Li Kui, Liu Ji-Wei, Zheng Jing*, Hu Ze-Lan*. Androglobin knockdown inhibits growth of glioma cell lines.International Journal of Clinical and Experimental Pathology, 2014. 7(5):2179-2184.（IF=1.738）

8.         Wang Hong-Tao, Zeng Lin, Zhang Xin, Li Kui, Zu Yong, Liu Ji-Wei, Liu Yong-Jie, Zhu Zhi-Chuan, Xiong Zhi-Qi, Zheng Jing*, Hu Ze-Lan*. Inactivation of β-catenin results in the reduction of postnatal body weight gain. Brain Research Bulletin, 2014. 100:55-60.（IF=2.974）

9.         Li Kui, Zhu Zhi-Chuan, Liu Yong-Jie, Liu Ji-Wei, Wang Hong-Tao, Xiong Zhi-Qi, Shen Xu, Hu Ze-Lan*, Zheng Jing*. ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299. International Journal of Clinical and Experimental Pathology, 2013. 6(11): 2460-2467.（IF=1.738）

10.     Zhu Yong-Chuan, Li Dan, Wang Lu, Lu Bin, Zheng Jing, Zeng Rong, and Xiong Zhi-Qi. Palmitoylation-dependent CDKL5-PSD-95 interaction regulates synaptic targeting of CDKL5 and dendritic spine development. Proceedings of the National Academy of Sciences of the United States of America, 2013. 110(22): 9118-9123. (IF=9.809)

11.     Zhu Zhi-Chuan, Li Kui, Xu Da-Feng, Liu Yong-Jie, Tang Hai-Liang, Xie Qing, Xie Li-Qian, Liu Ji-Wei, Wang Hong-Tao, Gong Ye, Hu Ze-Lan, Zheng Jing*. ZFX regulates glioma cell proliferation and survival in vitro and in vivo. Journal of Neuro-oncology, 2013. 112(1):17-25. (IF=2.787)

12.     Zhang Quan-Juan, Zhu Zhi-Chuan, Zheng Yong-Li, Cheng Jia-Gao, Zhang Na, Long Yi-Tao,  Zheng Jing*, Qian Xu-Hong*, Yang You-Jun*. A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite. Journal of the American Chemical Society, 2012. 134(45):18479-18482. (IF=11.444)

13.     Zhang Ling, Zheng Jing, Mai Hua-Ming, Zhu Ling, Zhang Lei, Zheng Jia-Wei. Efficacy of pingyangmycin injection for the treatment of cervical epidermoid cysts. International Journal of Clinical and Experimental Medicine, 2013. 6(1):81-83. (IF=1.422)

14.     Zheng Bin, Wang Chong, Wu Cong-Yu, Zhou Xue-Jiao, Lin Min, Wu Xiao-Chen, Xin Xiao-Zhen, Chen Xin, Xu Lin, Liu Hui, Zheng Jing, Zhang Jing-Yan, Guo Shou-Wu. Nuclease activity and cytotoxicity enhancement of the DNA intercalators via graphene oxide. Journal of Physical Chemistry, 2012. 116(29):15839−15846. (IF=4.835)

15.     Dai Hou-Ling, Zheng Jian-Bin, Lin Min, Zheng Jing, Zhou Fu-Sheng, Dong Xiao-Chun, Guo Lei, Liu Jian-Wen, Wen Ren. Synthesis and biological evaluation of noscapine analogues as microtubule-interfering agents. Acta Pharmaceutica Sinica, 2012. 47 (10): 1347−1357.

16.     Shen Peng-Cheng, Xu Da-Feng, Liu Ji-Wei, Li Kui, Lin Min, Wang Hong-Tao, Wang Rui, Zheng Jing*. TC10β/CDC42 GTPase activating protein is required for the growth of cortical neuron dendrites. Neuroscience, 2011. 199:589-597. (IF=3.327)

17.     Zheng Jing*, Xu Da-Feng, Wang Hong-Tao, Shen Peng-Cheng, Li Kui, Lin Min, Cao Xiao-Hua, Wang Rui. Neonatal exposure to fluoxetine and fluvoxamine altered spine density in mouse hippocampal CA1 pyramidal neurons. International Journal of Clinical and Experimental Pathology, 2011. 4(2):162-168. (IF=1.783)

18.     Ren Hong-Liu, Wang Chong, Zhang Jia-Li, Zhou Xue-Jiao, Xu Da-Feng, Zheng Jing, Guo Shou-Wu, Zhang Jing-Yan. DNA cleavage system of nanosized graphene oxide sheets and copper ions. ACS Nano, 2010. 4(12):7169-7174. (IF=12.033)

19.     Chen Qian, Zhu Yong-Chuan, Yu Jing, Miao Sheng, Zheng Jing, Xu Li, Zhou Yang, Li Dan, Zhang Chi, Tao Jiong, Xiong Zhi-Qi. CDKL5, a protein associated with rett syndrome, regulates neuronal morphogenesis via Rac1 signaling. Journal of Neuroscience, 2010. 30(38): 12777-12786. (IF=6.747)

20.     Liu Zan-Hua, Xu Da-Feng, Zhao Yong-Bo, Zheng Jing*. Non-syndromic mild mental retardation candidate gene CDKL3 regulates neuronal morphogenesis. Neurobiology of Disease, 2010. 39 (3):242-251. (IF=5.202)

21.     Jia Jie-Min, Chen Qian, Zhou Yang, Miao Sheng, Zheng Jing, Zhang Chi, Xiong Zhi-Qi. Brain-derived neurotrophic factor-tropomyosin-related kinase B signaling contributes to activity-dependent changes in synaptic proteins. Journal of Biological Chemistry, 2008. 283(30):21242-21250. (IF=4.6)

22.     Zheng Jing*, Shen Wan-Hua, Lu Ting-Jia, Zhou Yang, Chen Qian, Wang Zi, Xiang Ting, Chen Xiao-Jing, Zhu Yong-Chuan, Zhang Chi, Duan Shumin, Xiong Zhi-Qi. Clathrin-dependent endocytosis is required for TrkB-dependent Akt-mediated neuronal protection and dendritic growth. Journal of Biological Chemistry, 2008. 283(19):13280-13288. (IF=4.6)

23.     Chen Qian, Sheng He, Hu Xiao-Ling, Yu Jing, Zhou Yang, Zheng Jing, Zhang Chi, Duan Wen-Hu, Xiong Zhi-Qi. Differential roles of NR2A- and NR2B-containing NMDA receptors in activity-dependent brain-derived neurotrophic factor gene regulation and limbic epileptogenesis. Journal of Neuroscience, 2007. 27(3):542-552. (IF=6.747)

24.     Zhou Yang, Wu Hao, Li Shuai, Chen Qian, Cheng Xue-Wen, Zheng Jing, Takemori Hiroshi,  Xiong Zhi-Qi. Requirement of TORC1 for late-phase long-term potentiation in the hippocampus. PLOS ONE, 2006. 1:e16. (IF=3.534)

25.     Wang Lie-Cheng, Xiong Wei, Zheng Jing, Zhou Yang, Zheng Hui, Zhu Xue-Liang, Xiong Zhi-Qi, Zhou Zhuan. The timing of endocytosis following activation of a G-protein-coupled receptor in a sensory neuron. Biophysical Journal, 2006. 90(10):3590-3598. (IF=3.832)

备注：* 代表第一作者或者责任作者